Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set
نویسندگان
چکیده
منابع مشابه
Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set.
The design and evaluation of a set of universal primers and probe for the amplification of 16S rDNA from the Domain Bacteria to estimate total bacterial load by real-time PCR is reported. Broad specificity of the universal detection system was confirmed by testing DNA isolated from 34 bacterial species encompassing most of the groups of bacteria outlined in Bergey's Manual of Determinative Bact...
متن کاملMolecular diagnosis of African Swine Fever by a new real-time PCR using universal probe library.
A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the VP72 coding genome region. The detection range of the optimized UPL PCR technique was confirmed by ...
متن کاملDiagnosis of bacterial endophthalmitis by broad-range quantitative PCR.
AIM To measure the bacterial genome in ocular fluids and to analyse the clinical relevance of infectious endophthalmitis. METHODS Nineteen ocular fluid samples (eight aqueous humour and 11 vitreous fluid samples) were collected from 19 patients with suspected bacterial endophthalmitis. Fifty ocular samples from uveitis patients were also collected along with 40 samples from patients without o...
متن کاملMultiplex PCR detection of slowly-evolving trypanosomatids and neogregarines in bumblebees using broad-range primers.
AIMS The aims of this study were to design universal markers for different protozoan parasites of Bombus spp. based on the phylogenetic position of two important bumblebee parasites Crithidia bombi and Apicystis bombi. METHODS AND RESULTS Standard PCR and extraction techniques were used to amplify and sequence 18S rDNA. Phylogenetic analysis of the rDNA was performed in order to predict the p...
متن کاملA novel real-time quantitative PCR method using attached universal template probe.
A novel real-time quantitative polymerase chain reaction (PCR) method using an attached universal template (UT) probe is described. The UT is an approximately 20 base attachment to the 5' end of a PCR primer, and it can hybridize with a complementary TaqMan probe. One of the advantages of this method is that different target DNA sequences can be detected employing the same UT probe, which subst...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiology
سال: 2002
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-148-1-257